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DAFTAR ISI

HalamanHALAMAN JUDUL........................................................................................... i

HALAMAN PERSETUJUAN............................................................................ ii

MOTTO DAN PERSEMBAHAN...................................................................... iiiABSTRAK.......................................................................................................... iv

ABSTRACT........................................................................................................ v

KATA PENGANTAR.......................................................................................... vi

DAFTAR ISI....................................................................................................... viiiDAFTAR TABEL................................................................................................ x

DAFTAR GAMBAR.......................................................................................... xiDAFTAR LAMPIRAN....................................................................................... xii

BAB I PENDAHULUAN

1.1....................................................................................................LataB!la"an# ................................................................................... 1

1.$....................................................................................................R%m%&

an Ma&ala'................................................................................ (1.)....................................................................................................T%*%an

P!n!litian................................................................................... (

1.(....................................................................................................Man+a

at P!n!litian............................................................................... (

BAB II LANDASAN TEORI

$.1. Tin*a%an P%&ta"a....................................................................... ,A. Ta-a" Daa Catharanthus roseus/.................................... ,

B. E"&ta"&i Ma&!a&i............................................................. 0

C. Antia"t!i......................................................................... 2D. U*i A"tivita& Antia"t!i.................................................... 13

E. Ba"t!i U*i.......................................................................... 11

$.$. K!an#"a P!mi"ian.................................................................. 1)

A. K!an#"a T!4i................................................................... 1)

B. K!an#"a K4n&!-............................................................... 1($.). Hi-4t!&i&.................................................................................... 1(

BAB III METODE PENELITIAN

).1. J!ni& P!n!litian.......................................................................... 15

).$. T!m-at 6an 7a"t% P!n!litian.................................................... 15).). Ja68al P!n!litian....................................................................... 15


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).(. Ba'an......................................................................................... 1,

).5. P!alatan P!n!litian................................................................... 1,

).,. D!&ain P!n!litian....................................................................... 10).0. P!m%atan E"&ta" Da%n C. roseus.......................................... 10

).2. P!m%atan M!6ia...................................................................... 12).9. U*i A"tivita& Antia"t!i........................................................... 19

).13. P!n#%"%an :4na Hamat....................................................... $3

).11. I6!nti+i"a&i ;aia!l................................................................. $3

).1$. Anali&i& Stati&ti"...................................................................... $1

BAB I; HASIL DAN PEMBAHASAN

(.1. E"&ta"&i Da%n C. roseus.......................................................... $$(.$. P!n!nt%an A"tivita& Antimi"4a.............................................. $$

BAB ; KESIMPULAN DAN SARAN5.1. K!&im-%lan............................................................................... $9

5.$. Saan.......................................................................................... $9

DAFTAR PUSTAKA

LAMPIRAN


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BAB I

PENDAHULUAN

1.1 Latar Belakang

In64n!&ia m!%-a"an n!#aa am -!naa ta6i&i4nal 6a-at 6i-!tan##%n# *a8a"an

-!n##%naann


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tanaman?tanaman t!&!%t 6a-at 6i*a6i"an &!>aa alt!nati+ 4at?4atan "4m!&ial

Nia= $330/.

Um%mn

-!naan#. Batan# %lat= a#ian -an#"al !"ain#= t!-i ata= -!t%lan#an m!n


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B%a'ni&tin! aa

n4mal 6i 6alam 'i6%n# 6an -a6a "%lit &!"ita $5 ?)3 6ai 4an# 6!8a&a


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m!n

E. coliia&an

m!n

ata% m!lal%i "4nta" 6!n#an '!8an ata% 4an# CDC= $31$/. G!*ala -!n


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'amat >aa 6i&" 6i+%&i 6i %"% 6!n#an -!n##ai&= t!'a6a- 6a


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BAB II

LANDASAN TE%RI

2.1 TIN&AUAN PUSTA'A

A. Ta(ak Dara )Catharanthus roseus*

Ta-a" 6aa Catharanthus roseus/m!%-a"an tanaman '!a&!ma" il=

!"aaan#= 6an !am%t &an#at l!at. Da%n !&%&%n !'a6a-an=

!tan#"ai -!n6!"= m!man*an# %lat t!l% 6!n#an -an#"al &!%-a a*i 6an %*%n#

t%m-%l -an*an# $, >m= l!a 1) >m= 6an tan#"ai 6a%nnil N!8i!= $31)/.

Gama. 1.1.Ta-a" 6aa C. roseus/ 6i K4ta B!n#"%l%.

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Kla&i+i"a&i C. roseus

Kin#64m PlantaeT%m%'an/

S%"in#64m Tracheobionta T%m%'an !-!m%l%'/

Divi&i MagnoliophytaT%m%'an !%n#a/

S%-! Divi&i SpermatophytaM!n#'a&il"an i*i/

K!la& Magnoliopsida!"!-in# 6%a 6i"4til/

S% K!la& Asteridae

O64 Gentianales

Famili ApocynaceaeG!n%& Catharanthus

S-!&i!& Catharanthus roseus!."

K4m-4n!n anti"an"!=

6!n#an m!n##%na"an -!la%t t!t!nt%. E"&ta"&i t!#ant%n# -a6a t!"&t% 6an

"an6%n#an a'an 6alam t%m%'an. S!n


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"!la%tan aa m!!n6am &am-!l 6alam

-!la%t t!t!nt% 6!n#an ata% tan-a -!n#a6%"an. Ma&!a&i m!%-a"an m!t46!


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M!n%%t Ja8!t@ !t al. 199,/ 6an P!l>@a 6an C'an $339/= m!"ani&m! "!*a

antia"t!i 6i!6a"an m!n*a6i !m-at &!>aa %m%m=

>/. M!n#'amat &int!&i& -4t!in &!l a"t!i

S%'% 6an "4n&!nta&i tin##i @at "imia 6a-at m!n6!nat%a&i -4t!in


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"!a&aan -H/= -4t!n&i &%at% @at antimi"4a 6alam la%tan


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c. $is% Agar $i&&usin Test

M!t46! ini m!n##%na"an m!t46! >a"am "!ta& a8an

-!ti "!m%6ian m!n!m-at"an &%at% >a"am "!ta& am m!6ia= in4"%l%m

6an la*% 6i+%&i a'an antimi"4a. Ha&ilna

a/ :4na a6i"al


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"a4t!n4i6 !8ana "%ninn# m%6a &am-ai *in##a t%a. S. aureusini t!ma&%" 6alam

+amiliaMicrococcacea= m!%-a"an a"t!i #am -4&iti+ 6an !!nt%" "4"%& 6!n#an

6iam!t! 3=5?1=5 m ai" !-a&an#an ma%-%n !#!4m4l. Ba"t!i ini !&i+at

ti6a" m4til= 6a-at 'i6%- &!>aa a!4 6an ana!4 +a"%ltati+= -!t%m%'an -alin#

>!-at -a6a t!m-!at% )04C. P!m!nt%"an -i#m!n -alin# ai" -a6a a"t!i ini

a6ala' 6i &%'% "ama= @a 6an C'an 192,/.

Gama. 1.$. Ba"t!i S. aureus K!nn!t'= $31$/.

S. aureusm!%-a"an -!n

-!nim%nan nana' a"iat in+!"&i a"t!i/= &!6an#"an -a6a '!8an S.aureus

m!n

-4&t%la 6!matiti& a6an# "%lit/ -a6a an*in#= &!ta a&!& -a6a %n##a& N!8i!=$33$/.

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,*.Escherichia coli

E. colim!%-a"an -!n#'%ni %&%& !&a man%&ia 6an '!8an tin#"at tin##i

lainn@a 6an C'an 192,/.

Gama. 1.). Ba"t!iE. coli 7HO= $31)/

E. colit!ma&%" "!6alam +amiliEnterobacteriaceae= m!%-a"an a"t!i #am

n!#ati+= !&i+at ana!4 +a"%ltati+. Ba"t!i ini !!nt%" atan# -!n6!" 6!n#an l!a

"%an# 6ai 1=1?1=5 m 6an -an*an# &!"ita $=3?,=3 m= nilai -H 4-tim%mn


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2.2 'ERAN'A PEMI'IRAN

A. 'erangka Ter"

Gama. 1.(. K!an#"a t!4i -!n!litian.

B. 'erangka 'nse(

Dai lan6a&an t!4i 6iata& 6a-at 6i&%&%n &!>aa &"!mati& "!an#"a "4n&!- 6alam

-!n!litian ini


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2. HIP%TESIS

Hi-4t!&i& 6ai -!n!litian ini a6ala'

H1 E"&ta" 6a%n C. roseusmam-% m!n#'amat -!t%m%'an ata% m!mati"an

a"t!i S. aureus6an E. coli.

H3 E"&ta" 6a%n C. roseus ti6a" mam-% m!n#'amat -!t%m%'an ata%

m!mati"an a"t!i S. aureus6an E. coli.

15


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BAB III

MET%DE PENELITIAN

.1 &en"s Penel"t"an

J!ni& -!n!litian a analiti"= "!ta& &ain# 8'atman= -!n##ai&= '!m4&it4m!t!=

mi&"4&"4-= 6an alat?alat #!la& lainna"am dis% di&&usion test/. M!t46! %*i 6i+%&i >a"am dis%

16


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di&&usion test/ m!%-a"an &ala' &at% m!t46! aa &!an

&am-ai ( 'ai "!m%6ian 6i&ain#= -!la"%an ini 6ila"%"an &!lama ) 'ai. S!lan*%tn


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F4m%la&i m!6ia NA DIFCO -! lit! a6ala' 5 # a>t4 -!-t4n= ) #am bee&

e(tract= 5 # NaCL= 6an 15 # a>t4 a#a. S!an6an al%mini%m +4il. M!6ia ini 6i&t!il"an m!n##%na"an 4t4"la+ -a6a t!"anan 1=5

Atm= 1$14C &!lama 15 m!nit.

Me/"a Muller H"ntn Agar )MHA*M!6ia MHA 6!n#an "!t!alan "%an# l!i' , mm 6i%at 6!n#an >aa

m!niman# &t4" "!m%6ian 6ila%t"an 6alam a%a6!& &amil 6i-ana&"an 6an

6i6i&ti%&i"an -a6a ta%n# 6!n#an "!t!alan a8an -!ti &t!il 6!n#an 6iam!t! 6an m!"


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6iin4"%la&i"an "!6alam El!nm!

6iin"%a&i 6alam in"%at4 !#4a"am 6itii&"an

-a6a a#ian -in##i ta%n# &!lama 13 m!nit.

P!n#%*ian 6aa"am. In4"%la&i"an &%&-!n&i a"t!i -a6a nutrient agar


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-!t%m%'an a"t!i S. aureus 6anE.coli6i &!"ita >a"am. P!'it%n#an 6iam!t!

6a


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BAB. I:

HASIL DAN PEMBAHASAN

4.1 Ekstraks" Daun C. roseus

Ha&il !"ta"&i 133 # &!%" 6a%n C. roseus6!n#an 0533 ml !tan4l 9, 6i-!4l!'

!"&ta" -a6at !8ana 'i*a% -!"at &!an4>a-a S>'!++.Q B4!l/ m!n%n*%""an &!ma"in

21


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tin##i "4n&!nta&i &!ma"in !&a @4na a6i"aln


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n

S. aureus 1 $9 $2 $5 $1 10 $9

2 $9 $0 $5 $1 10 $9 $2 $5 $( $3 15 $9

Rata=rata 26


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Data 'a&il -!n!litian 6i4la' 6!n#an m!n##%na"an t!& S'a-i4?8il" "a!na

&am-!l )3/ %nt%" m!li'at a-a"a' @4na 'amat !"&ta" 6a%n ta-a" 6aa t!&!%t

!6i&ti%&i n4mal ata% ti6a". Ha&il -!n#4la'an 6ata 6a-at 6ili'at -a6a ta!l

!i"%t.

Ta!l $. T!& n4malita& t!'a6a- @4na 'amat !"&ta" 6a%n ta-a" 6aa 6!n#an

S'a-i4?8il" t!&t

Ba"t!i Si#.

:4na Hamat Staphylococcus aureus

Escherichia coli

3=00(

3=23)

Dai 'a&il t!& S'a-i4?8il" &am-!l )3/ 6i6a-at"an nilai &i#ni+i"an&i 3=00(

%nt%" Staphylococcus aureus6an 3=23) %nt%"Escherichia coli= 6imana nilai t!&!%t

l!i' !&a 6ai nilai - val%! 3=35/&!'in##a 6in


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Dai -!n#4la'an 6ata 6!n#an %*i t test idendepent6iata& 6i6a-at"an nilai !evene*s

test &or e+uality o& variance m!mili"i nilai &i#ni+i"an&i 3=932 3=35= &!'in##a

6iint!-!&ta&i"an &!a#ai &am-!l 6alam -!n!litian


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4l!' K%&ma

%niv!&al

Pa6a %m%mn


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a"tivita& -!n#'amatan -a6a a"t!i #am -4&iti+ l!i' !&a 6ai-a6a a"t!i #am

n!#ati+. Ba"t!i #am n!#ati+ l!i' an

&!n

6!n#an &!n


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t!'a6a- !!a-a a"t!i= 4l!' "a!na it% -!l% 6i>ai alt!nati+ -!n##anti -!ma"aian

antii4ti" 6!n#an 4at ta6i&i4nal.

P!n!litian ini m!n##%na"an antii4ti" t!ta&i"lin &!a#ai -!man6in# "a!na

t!ta&i"lin t!ma&%" antii4ti" !&-!"t%m l%a& aa m!n#'amat &int!&i&

-4t!in a"t!i P!l!@a 6an C'an= 1922/. Diam!t! 6a!a' 'amat antii4ti"

t!ta&i"lin )3 #6i&" m!n%%t NCCLS Nati4nal C4mmitt!! +4t Clini>al La4at4" JL= A6!l!# EA 199,/.Mi%robiologi /edo%teran Edisi %e,28.

Ja"ata K!64"t!an EGC.

Ja8!t@ E= M!lni>" JL= A6!l!# EA $335/.Mi%robiologi 3ntu% Pro&esi /esehatan.Ja"ata K!64"t!an EGC.

J4&46i84n64 S= UC 7a&a= A S4!'ian6i4 6an P S4!6am4n4 199,/.Ma0alah %edo%teran Indonesia 1 Per%embangan %uman terhadap antimi%roba

saat ini. I"atan D4"t! In64n!&ia= (,9/ (,0.

K!nn!t' T46a $33$/. The Control o& Microbial Gro'th. 7i&>4n&in Univ!&it< 4+

7i&>4n&in.

K!nn!t' T46a $31$/. )nline te(tboo% o& bacteriology 1 staphylococcus aureus and

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s. aureus desease. T'! Am!i>an& S4>i!t< +4 Mi>4i4l4#< E6%>ati4nal

7!&it! Mi>4 74l6. 888.t!xt44"4+a>t!i4l4#in!N!t= In>. 888.m!6i>in!n!t.>4m&ta-'?in+!>ti4nati>l!. 'tml.

Dia"&!& M!i $31).

Ma&niai P4!l4!n#an= An6iani= S%&an MN= I

30i $aya Antiba%teri E%stra% Etanol /ulit #atng #ungur !argerstoremia

spesiosa Pers" Terhadap Staphylococcus aureus $an Escherichia coli Secara

In Vitro. B4#4 Balai B!&a P!n!litian ;!t!in!.

N!8i! T4a $31)/.$unia Pertanian 1 Catharantus roseus- /lasi&i%asi Tanaman-

/lasi&i%asi Tapa% $ara Catharantus roseus"- Mor&ologi- Mor&ologi Tapa%$ara Catharantus roseus"- Tanaman :erba- Tanaman :erba Catharantus

roseus"- Tapa% $ara- Tapa% $ara Catharantus roseus". P!malin".

'tt-888."la&i+i"a&i?6an?m4+4l4#i?tanaman?ta-a"?6aa'Catharantusroseus/?N!8i!?T4a. 'tm.Dia"&!& A-il $31).

Nia In6iani $330/.A%tivitas Antiba%teri $aun Segugu Clerodindron serratum ;!." 7= Ma

UGM.

S%li&tia Gani&8ana $331/.4arma%ologi $an Terapi Edisi %e,9.Ja"ata

Fama"4l4#i FKUI.

S P4na%l&!lvam= C Pann!!&!lvam= K M%%#an= N At'i= K Kalim%t'%= an6 S

T'an#amani $31$/.Asian paci&ic 0ournal o& tro&ical biomedicine 1 synthesis

o& silver nanoparticles using leaves o& catharontus roseus linn.g. don and

their antiplasmodial activities. US Nati4nal Lia< 4+ M!6i>in! Nati4nalIn&tit%t!& 4+ H!alt'= $0/ 50(?523.

Ta%n Ma6a--a $31$/.$rugs- diseases and procedures 1 e. coli in&ections.M!6&>a-! R!+!!n>!. 888.!m!6i>in!.m!6&>a-!.>4mati>l!$10(25.

4v!vi!8. Dia"&!& M!i $31).

7a'i6 P 199)/.Medicinical and Aromatic Plant in Indonesia. Ban#"4" RAPAP%li>ati4n.

7inan4= Fa6ia@ D= Fa&ia@ S 190)/.E%stra%si- /romatogra&i- dan Ele%tro&oresis.

B4#4 Fa"%lta& T!"n4l4#i P!tanian= In&tit%t P!tanian B4#4.

7HO $31)/.:ealth topics 1 e. coli in&ections.74l6 H!alt' O#anitati4n.888.8'4.intt4-i>&!&>'!i>'ia>4liin+!>ti4n&. !n. 6ia"&!& M!i $31).

7%lan6ai NDM $335/.Perbandingan Metode E%stra%si #uah Mah%ota $e'aPhaleria macrocarpa" dan 30i To%sisitas Sub%ronis Pada Ti%us Putih.

B4#4 Fa"%lta& Mat!mati"a 6an Ilm% P!n#!ta'%an Alam= In&tit%t P!tanianB4#4.

7%lan O"taviani $31$/. Perbedaan E&e%tivitas $aya Antiba%teri Antara

/lorhe%sidin $i%lu%onat 2= $engan #erbagai /onsentrasi E%stra% #uah

Mah%ota $e'a Phaleria macrocarpa ;Sche&&.< #oerl" tin0auan terhadap

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Enterococcus &aecalis".4#


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LAMPIRAN 2

Has"l U!" Ant",akter" Ekstrak Daun C. roseusTerha/a(S. aureus/anE. coli

Se>araIn :"tr

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&a/?al Penel"t"an

Ta,le 1. Alkas" ?aktu (enel"t"an

N 'eg"atan

&un"

2912

&ul"

291

Agustus

291

Se(tem,er

291

1 $ ) ( 1 $ ) ( 1 $ ) ( 1 $ ) (

1 St%6i P!n6a'%l%an

$ P!n#a*%an J%6%l

) P!n


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P!n!litian

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