PENGOPTIMUMAN PEWARNAAN IMUNOHISTOKIMIA...
Transcript of PENGOPTIMUMAN PEWARNAAN IMUNOHISTOKIMIA...
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DAFTAR ISI
HALAMAN JUDUL ............................................................................................ .. i
HALAMAN PENGESAHAN ............................................................................... . ii
HALAMAN PERNYATAAN ............................................................................. iii
PRAKATA ........................................................................................................... iv
DAFTAR ISI ........................................................................................................ vi
DAFTAR GAMBAR ........................................................................................... ix
DAFTAR TABEL ................................................................................................ . x
DAFTAR LAMPIRAN ........................................................................................ xi
ARTI LAMBANG DAN SINGKATAN ............................................................. xii
INTISARI ............................................................................................................ xiii
ABSTRACT ........................................................................................................ xiv
BAB I PENDAHULUAN .................................................................................... . 1
I.1. Latar Belakang ..................................................................................... . 1
I.2. Perumusan Masalah .............................................................................. . 3
I.3. Tujuan Penelitian ................................................................................... . 3
I.4. Keaslian Penelitian ............................................................................... . 3
I.5. Manfaat Penelitian ................................................................................ . 4
BAB II TINJAUAN PUSTAKA ......................................................................... . 5
II.1. Tinjauan Pustaka ................................................................................. . 5
II.1.A. Serabut Saraf Intraepidermal ................................................. . 5
II.1.B. Imunohistokimia .................................................................... . 8
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II.1.C. Perbandingan Metode Pewarnaan Imunohistokimia Dengan
Antibodi Anti PGP 9.5 ...................................................................... 11
II.2. Landasan Teori .................................................................................... 16
II.3. Kerangka Teori ................................................................................... 17
II.4. Kerangka Konsep ................................................................................ 18
BAB III METODE PENELITIAN ....................................................................... 19
III.1. Jenis dan Rancangan Penelitian .......................................................... 19
III.2. Variabel Penelitian ............................................................................. 19
III.3. Definisi Operasional ........................................................................... 19
III.4. Bahan dan Alat Penelitian ................................................................. 20
III.4.A. Subjek Penelitian .................................................................. 20
III.4.B. Bahan Penelitian ................................................................... 20
III.4.C. Alat Penelitian ...................................................................... 20
III.5. Jalannya Penelitian ............................................................................. 22
III.6. Analisis Hasil Penelitian .................................................................... 28
BAB IV HASIL DAN PEMBAHASAN ............................................................. 29
IV. 1. Hasil Penelitian ................................................................................. 29
Pengoptimuman Pewarnaan Imunohistokimia Dengan Antibodi Anti PGP
9.5 ................................................................................................................ 29
IV.2. Pembahasan ....................................................................................... 34
BAB V KESIMPULAN, SARAN DAN RINGKASAN ..................................... 37
V.1. Kesimpulan ......................................................................................... 37
V.2. Saran ................................................................................................... 37
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V.3. Ringkasan ........................................................................................... 38
V.3.A. Latar Belakang........................................................................ 38
V.3.B. Landasan Teori ...................................................................... 38
V.3.C. Cara Penelitian ........................................................................ 39
V.3.D. Hasil ........................................................................................ 40
V.3.D. Kesimpulan ............................................................................ 40
DAFTAR PUSTAKA .......................................................................................... 41
LAMPIRAN .......................................................................................................... 50
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DAFTAR GAMBAR
Gambar 2.1. Struktur lapisan kulit dan reseptor-reseptor sensoris yang terlibat
dalam sensasi sentuhan ...................................................................... . 6
Gambar 2.2. Lokasi inervasi dan penjuluran serabut saraf intraepidermal kornu
dorsalis ............................................................................................. . 7
Gambar 2.3. Pewarnaan imunohistokimia secara langsung (direk) ..................... . 9
Gambar 2.4. Pewarnaan imunohistokimia secara tidak langsung (indirek) ......... 10
Gambar 2.5. Kerangka teoritis deteksi serabut saraf intraepidermal dengan metode
imunohistokimia ................................................................................. 17
Gambar 2.6. Kerangka konsep penelitian pengoptimuman pewarnaan
imunohistokimia dengan antibodi anti PGP 9.5 untuk deteksi serabut
saraf intraepidermal dari biopsi kulit ................................................ 18
Gambar 3.1. Lokasi biopsi kulit kaki mencit ....................................................... 24
Gambar 3.2. Aturan penghitungan serabut saraf intraepidermal ......................... 28
Gambar 4.1. Hasil Pengoptimuman Tahap I ........................................................ 30
Gambar 4.2. Gambaran serabut saraf intraepidermal dengan antibodi anti PGP 9.5
........................................................................................................ 31
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DAFTAR TABEL
Tabel 2.1. Perbandingan Metode Pewarnaan Imunohistokimia Dengan Antibodi
Anti PGP 9.5 ..................................................................................... 13
Tabel 4.1 Hasil Pengoptimuman Pewarnaan Imunohistokimia Dengan Antibodi
Anti PGP 9.5 .................................................................................... 33
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DAFTAR LAMPIRAN
Ethical Clearance.................................................................................................. 50
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ARTI LAMBANG DAN SINGKATAN
BB Berat Badan
DAB Diaminobenzidine
EFNS European Federation of Neurological Societies
HRP Horseradish peroxidase
LPPT Lembaga Pengujian dan Penelitian Terpadu
NA Numerical aperture
PBS Phosphate Buffered Saline
PGP 9.5 Protein gene product 9.5
UPHP Unit Pengembangan Hewan Percobaan