panduan patologi kimia 2015
Transcript of panduan patologi kimia 2015
PANDUAN
PERKHIDMATAN PATOLOGI KIMIA
HOSPITAL SULTANAH AMINAH
JOHOR BAHRU
2015
PANDUAN PERKHIDMATAN PATOLOGI KIMIA
HOSPITAL SULTANAH AMINAH JOHOR BAHRU VERSI 1 (2015)
KANDUNGAN
M/S
Pengenalan
1
Lokasi Makmal 1
Skop Perkhidmatan 1
Waktu Perkhidmatan
Hubungi Makmal
1
1
Informasi Pegawai 2
Senarai Ujian yang dijalankan di makmal Patologi Kimia, HSAJB 3-10
Senarai Ujian yang dirujuk ke makmal luar 11-21
Lampiran 22
Lampiran 1: Senarai Ujian STAT 23
Lampiran 2: Jadual Penghantaran Spesimen ke makmal rujukan 24
Lampiran 3: Panduan Bekas Spesimen 25
Lampiran 4: Panduan Order of Draw For Multiple Tube Collections 26
Lampiran 5: Panduan ujian Renin dan Aldosterone 27-28
Lampiran 6: Panduan Ujian Inborn Error of Metabolism 29-31
Lampiran 7: Borang-Borang 32-42
1
PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
PERKHIDMATAN PATOLOGI KIMIA
PENGENALAN
Unit Patologi Kimia menawarkan perkhidmatan analisa spesimen dan konsultasi keputusan
untuk tujuan membantu perawatan pesakit di Hospital Sultanah Aminah, Johor Bahru. Unit ini juga
menjadi makmal rujukan bagi ujian-ujian khas tertentu untuk seluruh Negeri Johor.
LOKASI
Tingkat Bawah, Bangunan Teratai
Hospital Sultanah Aminah
SKOP PERKHIDMATAN
Perkhidmatan Patologi Kimia meliputi analisa dan interpretasi perubahan biokimia dalam darah
dan cecair badan untuk tujuan penyaringan, diagnosa dan pemantauan penyakit. Perkhidmatan
dibahagikan kepada beberapa seksyen iaitu: main chemistry, immunoassay, special protein, diabetic
tests, urine and body fluids, miscellaneous tests dan seksyen dadah. Unit ini juga mengendalikan
pemprosesan dan penghantaran spesimen bagi ujian biokimia ke makmal-makmal rujukan sekiranya
ujian tidak ditawarkan di sini.
WAKTU PERKHIDMATAN
24 jam sehari
Setiap hari termasuk cuti umum
HUBUNGI MAKMAL
Makmal Patologi Kimia
No Tel: 07-2231666 (ext 2630)
Fax: 07-2231803
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
INFORMASI PEGAWAI
NAMA PEGAWAI
NO TELEFON
Dr Shahannim Binti Izham
Pakar Patologi Kimia
Ketua Unit Patologi Kimia
No Tel: 07-2231666 (ext 2633)
Dr Yong Pei Wen
Pegawai Perubatan
No Tel: 07-2231666 (ext 2630)
Encik Abdul Halim bin Gahar
Pegawai Sains Kanan
Ketua Pegawai Sains
No Tel: 07-2231666 (ext 2634)
Pn Noor Hayati A. Kadir
Pegawai Sains Kanan
Y/M Seksyen Dadah
No Tel: 07-2231666 (ext 2633)
Pegawai-pegawai Sains:
Pn Deviga Balakersnan
Pn Nooraishah Pairan
Pn Suhana Razali
Encik Khairul Anuar Abu Kasim
No Tel: 07-2231666 (ext 2630)
Juruteknologi Makmal Perubatan Kanan:
Pn Ashila Jaafar
En Sudin Suni
No Tel: 07-2231666 (ext 2630)
SENARAI UJIAN YANG DIJALANKAN DI MAKMAL PATOLOGI KIMIA, HSAJB
NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
1 Alanine Transaminase (ALT) Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
2 Albumin Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
CSF Plain container/Bijou bottle
1 ml 1 hour (STAT)
3 Alkaline Phosphatase (ALP) Main Chemistry Blood Li Heparin 4 mls 4 hours
4 Alpha feto protein (AFP) Immunoassay Blood Li Heparin 4 mls 3 working days Requesting specialist’s signature is required
5 Ammonia Main Chemistry Blood EDTA 2.5 mls 1 hour (STAT)
Send EDTA tube in ice
6 Amylase Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine Urine container 5 mls 1 hour (STAT) 4 hours (routine)
7 Apt Test Miscellaneous Blood in gastric aspirate/stool
Universal/urine container
>1 ml 1 hour
8 Arterial blood gas (ABG) 1) pH 2) pO2 3) pCO2 4) HCO3
STAT Blood Heparinized syringe
0.5-1 mls 30 minutes (STAT) Send sample in ice
9 Aspartate Transaminase (AST) Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
10 Beta Human Chorionic Gonadotrophic (BHCG)
Immunoassay Blood Li Heparin 4 mls 3 working days
11 Bilirubin, Total Main Chemistry Blood Li Heparin 4 mls* 1 hour (STAT) 4 hours (routine)
12 Bilirubin, Direct Main Chemistry Blood Li Heparin 4 mls* 1 hour (STAT) 4 hours (routine)
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SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
13 Bilirubin, Indirect Main Chemistry - - - 1 hour (STAT) 4 hours (routine)
Calculated from total bilirubin and direct bilirubin
14 Body fluid for biochemistry 1) Total protein 2) Albumin 3) LDH 4) Amylase 5) Glucose
Main Chemistry Body fluid Universal container
4 mls 4 hours
15 CA 125 Immunoassay Blood Li Heparin 4 mls 3 working days Requesting specialist’s signature is required
16 Carcinoembryonic Antigen (CEA) Immunoassay Blood Li Heparin 4 mls 3 working days Requesting specialist’s signature is required
17 Cardiac enzymes (CE) 1) CK 2) AST 3) LDH
Main Chemistry Blood Li Heparin 4 mls 4 hours
18 C-Reactive Protein (CRP) Main Chemistry Blood Li Heparin 4 mls 4 hours
19 Complement 3 (C3) Special Protein Blood Li Heparin 4 mls 2 days
20 Complement 4 (C4) Special Protein Blood Li Heparin 4 mls 2 days
21 Calcium Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
22 Cerebrospinal Fluid (CSF) for biochemistry
1) Appearance 2) Protein 3) Glucose 4) Lactate
Main Chemistry CSF Plain container/Bijou bottle
1 ml
1 hour (STAT)
Send CSF sample immediately to lab.
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
23 Chloride Main Chemistry
Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
CSF Plain container/Bijou bottle
1 ml 1 hour (STAT)
Send CSF sample immediately to lab.
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
24 Cholinesterase Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
25 Cortisol Immunoassay Blood Li Heparin 4 mls 3 working days
26 Creatine Kinase (CK) Main Chemistry Blood Li Heparin 4 mls 4 hours
27 Creatinine Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
28 Estradiol Immunoassay Blood Li Heparin 4 mls 5 working days
29 Ferritin Immunoassay Blood Li Heparin 4 mls 3 hours (STAT*) 3 working days
*STAT testing for severe dengue cases
30 Folate Immunoassay Blood Plain tube/ Li Heparin
4 mls 3 working days
31 Follicle Stimulating Hormone (FSH)
Immunoassay Blood Li Heparin 4 mls 5 working days
32 Free Triiodothyronine (FT3) Immunoassay Blood Li Heparin 4 mls 3 working days
33 Free Thyroxine (FT4) Immunoassay Blood Li Heparin 4 mls 3 working days
34 Gamma Glutamyl Transferase (GGT)
Main Chemistry Blood Li Heparin 4 mls 4 hours
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
35 Globulin Main Chemistry - - - 4 hours Calculated from total protein and albumin
36 Glucose Main Chemistry Blood Fluoride tube 2 mls 1 hour (STAT) 4 hours (routine)
37 HBA1C Miscellaneous Blood EDTA tube 2.5 mls 3 working days
38 Immunoglobulin A (IgA) Special protein Blood Li Heparin 4 mls 2 working days
39 Immunoglobulin G (IgG) Special protein Blood Li Heparin 4 mls 2 working days
40 Immunoglobulin M (IgM) Special protein Blood Li Heparin 4 mls 2 working days
41 Iron, Total Main Chemistry Blood Li Heparin 4 mls 2 working days
42 Iron profile/Study 1) Total iron 2) Unbound iron binding
capacity (UIBC) 3) Total iron binding
capacity (TIBC)
Main Chemistry Blood Li Heparin 4 mls 2 working days TIBC is calculated from Total iron and UIBC
43 Lactate Main Chemistry Blood
Fluoride tube 4 mls 1 hour (STAT)
44 Lactate Dehydrogenase, (LDH) Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
45 Leutinizing Hormone (LH) Immunoassay Blood Li Heparin 4 mls 5 working days
46 Lipid Profile 1) Total Cholesterol, (TC) 2) HDL-Cholesterol 3) LDL-Cholesterol 4) Triglycerides (TG)
Main Chemistry Blood Li Heparin 4 mls 2 working days LDL-Cholesterol is calculated from TC, HDL-C and TG
47 Liver Function Test 1) Total Protein 2) Albumin 3) Globulin 4) Total bilirubin 5) ALP 6) ALT
Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT*) 4 hours (routine)
* STAT request is offered for acute liver failure, pre-eclampsia and severe dengue cases only. Call Medical Officer/Chemical Pathologist on call for urgent requests for other indications
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
48 Magnesium (MG) Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
49 Myoglobin Immunoassay Blood Li Heparin 4 mls 4 hours (routine)
50 Osmolality Miscellaneous Blood Plain tube 2 mls 3 hours
Urine Urine Container 2 mls 3 hours
51 Phosphate Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
52 Potassium Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
53 Pre-eclampsia profile (PE profile) 1) Sodium 2) Potassium 3) Chloride 4) Urea 5) Creatinine 6) Uric acid 7) ALT 8) AST
Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
54 Prolactin Immunoassay Blood Li Heparin 4 mls 3 working days
55 Progesterone Immunoassay Blood Li Heparin 4 mls 5 working days
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
56 Prostate Specific Antigen (PSA) Immunoassay Blood Li Heparin 4 mls 3 working days Requesting specialist’s signature is required
57 Renal Profile 1) Sodium 2) Potassium 3) Chloride 4) Urea 5) Creatinine
Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
58 Rheumatoid Factor Special Protein Blood Li Heparin 4 mls 2 days
59 Sodium Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
60 Stool occult blood Miscellaneous Stool Urine container 1 gm 3 hours
61 Testosterone Immunoassay Blood Li Heparin 4 mls 3 working days
62 Thyroid function test 1) TSH 2) Free T4
Immunoassay Blood Li Heparin 4 mls 3 working days
63 Thyroid Stimulating Hormone, TSH
Immunoassay Blood Li Heparin 4 mls 3 working days
64 Total Iron Binding Capacity, TIBC Main Chemistry - - - 2 days Calculated from Total Iron and UIBC
65 Total Protein Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
66 Total Serum Bilirubin (TSB) 1) Total bilirubin 2) Direct Bilirubin
Main Chemistry Blood Li Heparin 4 mls (*400-600ul in microtainer tube for pediatric patients)
1 hour (STAT) 4 hours (routine)
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
67 Troponin T STAT Blood Li Heparin 4 mls 1 hour (STAT)
68 Unbound Iron Binding Capacity, UIBC
Main Chemistry Blood Li Heparin 4 mls 2 days
69 Urea Main Chemistry Blood Li Heparin 4 mls 1 hour (STAT) 4 hours (routine)
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
70 Uric acid Main Chemistry Blood Li Heparin 4 mls 4 hours
Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
71 Urine microalbumin Main Chemistry Urine random/24 hours
Urine container/ 24hr urine container
5 mls/ 24 hours collection
4 hours
72 Urine albumin Creatinine Ratio (ACR)
Main Chemistry urine Urine Container 5 mls random 4 hours ACR is calculated from urine microalbumin and urine creatinine
73 Urine hemoglobin Miscellaneous urine Urine container 2 mls 3 hours
74 Urine myoglobin Miscellaneous urine Urine container 2 mls 3 hours
75 Urine paraquat Miscellaneous urine Urine container 5 mls 1 hour
76 Urine reducing sugar Miscellaneous urine Urine container 2 mls 3 hours
77 Urine pregnancy test Miscellaneous urine Urine container 2 mls 3 hours
78 Vitamin B 12 Immunoassay Blood Li Heparin 4 mls 3 working days
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NO TESTS SECTION TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
LAB TURN AROUND TIME
REMARK
79 Morphine screening and confirmation
Drug Urine Urine container 20 mls 5 working days Use form UDP 1 (PINDAAN) *PER.PAT301 form is strictly for inpatient only
80 Cannabinoid screening and confirmation
Drug Urine Urine container 20 mls 5 working days
81 Amphethamine type stimulant (ATS) screening and confirmation
Drug Urine Urine container 20 mls 14 working days
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10
SENARAI UJIAN PATOLOGI KIMIA YANG DI RUJUK KE MAKMAL LUAR
NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
1. Acid alpha glucosidase Biochemistry unit, IMR
Dried blood spot Whatmann 903 filter paper
3 circles of blood spot
15 working days IEM form
2. Adenocorticotrophic Hormone (ACTH)
HKL Blood EDTA in ice 3 mls 5 working days
3. Aldosterone Putrajaya Blood EDTA 3 mls 6 weeks Send sample rapidly to lab but NOT in ice
Use different tubes for Renin and aldosterone.
Only single form is required for aldosterone & renin.
4. Alpha-1-acid glycoprotein
Ampang Blood Plain tube 4 mls 10 working days
5. Alpha-1-antitrypsin, Quantitation HKL Blood Plain tube 3 mls 5 working days
6. Alpha-1-antitrypsin, Phenotyping
Molecular and Protein Unit, IMR
Blood Plain tube 4 mls 10 working days IMR/UMDP/01 form
7. Alpha-2-macroglobulin
Ampang Blood Plain tube 4 mls 10 working days
8. Amino acid Biochemistry Unit, IMR
CSF Universal container
0.5 mls 10 working days IEM form
Blood Lithium heparin 4 mls
Urine Urine container 5 mls
9. Androstenedione Putrajaya Blood Plain tube 3 mls 6 weeks
10. Apolipoprotein A HKL Blood Plain tube 3 mls 20 working days
11. Apolipoprotein B HKL Blood Plain tube 3 mls 20 working days
12. Beta-2-microglobulin Ampang Blood Plain tube 4 mls 10 days
* Use PER PAT 301 form unless otherwise stated.
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NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
13. Biotinidase Enzyme Activity Biochemistry Unit, IMR
Dried blood spot in filter paper
Whatmann 903 Filter paper
3 circles of Dried blood spots
10 working days
14. CA 15-3 HKL Blood Plain tube 3 mls 2 working days
15. CA 19-9 HKL Blood Plain tube 3 mls 2 working days
16. Caeruloplasmin HKL Blood Plain tube 3 mls 5 working days
17. Carnitine, Total and Free, plasma Biochemistry Unit, IMR
Blood Lithium heparin 4 mls 5 working days IEM form
18. Cathecholamine Putrajaya Urine 24 hours urine container with 10 mls of 25% HCL
24 hours urine collection
6 weeks
19. Copper HKL Blood Plain tube 3.5 mls 10 working days
Urine 24 hour urine container without preservative
24 hour urine collection
10 working days
20. Cortisol, Urine Free Cortisol HKL Urine 24 hour urine container without preservative
24 hour urine collection
3 working days
21. C-Peptide HKL Blood Plain tube 3 mls 5 working days
22. CSF Electrophoresis (oligoclonal bands)
Molecular and Protein Unit, IMR
Paired blood and CSF samples
Plain tube/ sterile container
4 mls blood 1 ml CSF
10 working days IMR/UMDP/01 form
23. Dehydroepiendosterone Sulphate (DHEAS)
Diabetes and endocrine unit, IMR
Blood Plain tube 3 mls 4 weeks
24. Delta-Amino Levulinic Acids (D-ALA)
Biochemistry Unit, IMR
Urine 24 hour 24 hour urine container with 10 mls glacial acetic acid
24 hour urine collection
15 working days IEM form
25. Free light chain kappa & lambda Molecular and Protein Unit, IMR
Blood Plain tube
4 mls 5 working days IMR/UMDP/01 form
26. Fructosamine Ampang Blood Plain tube 4 mls 10 working days
* Use PER PAT 301 form unless otherwise stated.
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12
NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
27. Galactose, Total/Screening, blood spot
Biochemistry Unit, IMR
Dried blood spot Whatmann 903 Filter paper
3 circles of dried blood spots
5 working days IEM form
28. Galactose-1-phosphate Uridyl Transferase (GALT/G1PUT), blood spot
Biochemistry Unit, IMR
Dried blood spot Whatman 903 Filter paper
3 circles of dried blood spots
5 working days IEM form
29. Glycosaminoglycan (GAG), urine Biochemistry Unit, IMR
First morning urine
Urine container 5 mls 10 working days IEM form
30. Growth Hormone Diabetes and Endocrine Unit, IMR
Blood Plain tube 3 mls 4 weeks
31. Haptoglobulin HKL Blood Plain tube 3 mls 5 working days
32. Homocysteine HKL Blood Plain tube in ice 3 mls 20 working days
33. Ig E, Specific Allergy, IMR Blood Plain tube 3 mls 5 working days
34. Ig E, Total Allergy, IMR Blood Plain tube 3 mls 5 working days
35. Insulin HKL Blood Plain tube 3 mls 10 working days
36. Insulin Like Growth Factor-1 (IGF-1)
Diabetes and Endocrine Unit, IMR
Blood Plain tube 3 mls 4 weeks
37. Intact Parathyroid hormone Diabetes and Endocrine Unit, IMR
Blood EDTA 2.5 mls 4-5 weeks
38. Lead Toxicology and Pharmacology, IMR
Blood Lithium heparin 2 mls 14 working days
39. Lipoprotein (a) electrophoresis Molecular and Protein Unit, IMR
Blood Plain tube 4 mls blood 10 working days IMR/UMDP/01 form
40. Lithium Permai Blood Plain tube 3 mls Same working day (STAT 1 hour)
Need appointment for STAT request
41. Lysosomal enzymes screening Biochemistry Unit, IMR
Dried blood spot Whatmann 903 Filter paper
3 circles of dried blood spots
10 working days IEM form
42. Mercury, urine Toxicology and Pharmacology, IMR
Urine Urine container 10 mls 14 working days
* Use PER PAT 301 form unless otherwise stated.
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NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
43. Metabolic Screening, blood spot (Test for amino acids and acylcarnitines)
Biochemistry Unit, IMR
Dried blood spot Whatmann 903 Filter paper
3 circles of dried blood spots
3 working days IEM form
44. Metabolic Screening, urine(Test for amino acids and acylcarnitines)
Biochemistry Unit, IMR
Urine, random Urine container 2 mls 10 working days IEM form
45. Mucopolysaccharides Screening (MPS), urine
Biochemistry Unit, IMR
First morning urine
Urine container 5 mls 10 working days IEM form
46. Oligosaccharide & tetraglucoside, urine
Biochemistry Unit, IMR
First morning urine
Urine container 5 mls 15 working days IEM form
47. Organic Acids, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 5 working days IEM form
48. Orotic Acids, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 5 working days IEM form
49. Phytanic Acid Biochemistry Unit, IMR
Blood EDTA/Lithium heparin
4 mls 10 working days IEM form
50. Pipecolic acid Biochemistry Unit, IMR
Blood Lithium heparin 4 mls 10 working days IEM form
51. Porphobilinogen, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 15 working days IEM form
52. Porphyrin, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 15 working days IEM form
53. Pre-albumin Ampang Blood Plain tube 4 mls 10 working days
54. Protein electrophoresis Molecular and Protein Unit, IMR
Blood Plain tube 4 mls 30 working days IMR/UMDP/01 form
Urine Urine container 24 hour collection preferable
30 working days
* Use PER PAT 301 form unless otherwise stated.
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NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
55. Renin Putrajaya Blood EDTA 3 mls 6 weeks Send sample rapidly to lab but NOT in ice
Use different tubes for Renin and aldosterone.
Only single form is required for aldosterone & renin.
56. Sialic Acid, Total and Free Biochemistry Unit, IMR
First morning urine
Urine container 5 mls 15 working days IEM form
57. S-Sulphocysteine, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 10 working days IEM form
58. Succinylacetone, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 15 working days IEM form
59. Sweat Test HKL Not applicable Need approval from pediatric chest specialist. (call ext 6902, HKL for appointment)
60. Thyroglobulin Diabetes and endocrine unit, IMR
Blood Plain tube 3 mls 4-5 weeks
61. Transferrin HKL Blood Plain tube 3 mls 5 working days
62. Transferin Isoform for CDG Type I and Type II screening
Molecular and Protein Unit, IMR
Blood Plain tube 5 mls blood
15 working days IMR/UMDP/01 form
63. Transferin Isoform for CDG type II confirmatory profiling
Molecular and Protein Unit, IMR
Blood Plain tube 5 mls blood
20 working days IMR/UMDP/01 form
64. Uroporphobilinogen, urine Biochemistry Unit, IMR
Urine, random Urine container 5 mls 10 working days
* Use PER PAT 301 form unless otherwise stated.
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NO TESTS HOSPITAL TYPE OF SPECIMEN
SPECIMEN CONTAINER
VOLUME OF SPESIMEN
REFERRAL LAB TURN AROUND TIME
REMARK*
65. Very Long Chain Fatty Acid (VLCFA), plasma/serum
Biochemistry Unit, IMR
Blood EDTA/Lithium heparin
3 mls 10 working days
66. Vitamin D Putrajaya Blood Plain tube 3 mls 6 weeks
67. 5-Hydroxyindol-acetic acid (5-HIAA)
Biochemistry, IMR
Urine, 24 hours 24 hour urine container with 10 mls 25% HCL
24 hours collection
10 working days
68. 17-Hydryoxyprogesterone Diabetes and endocrine unit, IMR
Blood Plain tube 4 mls 4 to 5 weeks
* Use PER PAT 301 form unless otherwise stated.
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SENARAI UJIAN GENETIC/MOLECULAR
Notes:
All specimen for Genetic/Molecular Testing will be send to Unit of Molecular Diagnostics and Protein (UMDP), Specialized Diagnostic Centre, IMR
Use Request Form for Molecular Diagnostic Services (IMR/UMDP/o2, version 4.2)
Consent from patient or legal guardian is required.
Requesting specialist signature is required.
All Samples are blood in EDTA tubes or blood spots on Whatman 903 Filter paper unless otherwise stated
Volume required is 1-2 x 2.5 mls blood in EDTA tubes or 4 circles of dried blood spots unless otherwise stated.
All Laboratory TAT is 30 working days unless otherwise stated
NO DISEASE (TEST NAME)
METABOLIC DISORDERS
A) Amino Acids Metabolism Disorders
1. 2. 3. 4. 5. 6.
Maple Syrup Urine Disease (BCKDHA, BCKDHB, DBT, DLD gene sequence) Non Ketotic Hyperglycinemia (AMT , GCSH, GLDC gene sequence, GLDC Del/Dup analysis) N-Acetylglutamate Synthase Deficiency (NAGS gene sequence) Urea Cycle Disorder, Carbamoyl Phosphate Synthetase 1 Deficiency (CPS1 gene sequence) *LTAT: 60 days Urea Cycle disorders, Ornithine Transcarbamylase Deficiency (OTC gene sequence, OTC Del/Dup analysis) Urea cycle disorders- Type I Citrullinemia/Argininosuccinate Synthase Deficiency (ASS1 gene sequence) Urea cycle disorder-Arginosuccinic aciduria/Argininosuccinate Lyase
Deficiency (ASL gene sequence)
7. 8. 9. 10. 11. 12. 13. 14.
Lysinuric Protein Intolerance (SLC7A7 gene sequence) Classical Homocysteinuria (CBS gene sequence) Isolated Methylmalonic Acidemia (MMAA, MMAB, MUT gene sequence) Methylmalonic Aciduria and Homocystinuria Type C (MMACHC gene sequence) Methylmalonic Aciduria and Homocystinuria Type D (MMADHC gene sequence) Methylmalonyl-CoA Epimerase Deficiency (MCEE gene sequence) Methylenetetrahydrofolate Reductase Deficiency (MTHFR gene sequence) Glutaric Aciduria Type 1 (GCDH gene sequence)
B) Fatty acid metabolism disorders
16. 17. 18.
Fatty acid oxidation defects-Carnitine Palmitoyltransferase 1 Deficiency (CPT1A gene sequence) Fatty acid oxidation defects-Carnitine Palmitoyltransferase 2 Deficiency (CPT2 gene sequence) Carnithine-Acylcarnitine Translocase Deficiency (SLC25A20 gene sequence)
19. 20. 21.
Carnithine Uptake Deficiency (OCTN2 gene sequence) Long-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (HADHA gene sequence) Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (HADH gene sequence)
17
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, HSA
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01
5
C) Carbohydrate metabolism
22. 23.
Classical Galactosemia (GALT gene sequence) Galactokinase Deficiency (GALK1 gene sequence)
24. 25.
Fructose-1,6-Bisphosphatase Deficiency (FBP1 gene sequence) Galactosemia Epimerase Deficiency (GALE gene sequence)
D) Purine & Pyrimidine Metabolism Disorders
26. 27.
Dihydropyriminidase Deficiency (DPYS gene sequence) Purine Nucleoside Phosphorylase Deficiency (PNP gene sequence)
28. 29.
Lesch-Nyhan Syndrome (HPRT1 gene sequence) Hereditary Orotic Aciduria (UMPS gene sequence)
E) Others
30. 31. 32. 33.
Urea cycle disorder-Type II citrullinemia/Citrin Deficiency (SLC25A13 gene sequence) Tyrosine Hydroxylase Deficiency (TH gene sequence) Aromatic Amino Acid Decarboxylase Deficiency (DDC gene sequence) Ethylmalonic Encephalopathy (ETHE1 gene sequence)
34. 35. 36. 37. 38.
Pompe Disease (GAA gene sequence) Pyruvate Dehydrogenase Deficiency (PDHA1 gene sequence) Alpha-1-antitrypsin Deficiency (SERPINA1 gene sequence) Sulfite Oxidase Deficiency (SUOX gene sequence) X-linked Adrenoleukodystrophy (ABCD1 gene sequence)
MITOCHONDRIAL DISORDERS
39. 40. 41. 42.
Leigh Syndrome (8993 hotspot/mtATP5 gene sequence*,SURF1 gene sequence, mtDNA full panel) *LTAT 14 days for hotspot Mitochondrial DNA Deletion Syndromes – Pearson Syndrone, Kearn-Sayre Syndrome (KSS)*, Chronic Progressive External Ophthalmopegia (CPEO)* Test for mtDNA genes deletion/ duplication analysis * accepted samples: muscle biopsy in sterile container/ 10-20 mls of early morning urine/1-2 X 2.5mL blood EDTA Leber Hereditary Optic Neuropathy (LHON) Syndrome Test for mtDNA genes sequence (LHON Panel) Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-Like Episodes (MELAS) Syndrome (3243 hotspot/ mt-tRNA Leu sequence, full panel) * accepted samples: muscle biopsy in sterile container/ 20 mls of early morning urine/1-2 X 2.5mL blood EDTA **LTAT for hotspot: 14 working days
43. 44. 45. 46. 47. 48. 49.
Myoclonic Epilepsy with Ragged-Red Fibers (MERRF) Syndrome (8344 hotspot/Test for mt-tRNA Lys sequence * accepted samples: muscle biopsy in sterile container/ 20 mls of early morning urine/1-2 X 2.5mL blood EDTA **LTAT : 14 working days Neuropathy, Ataxia and Retinitis Pigmentosa (NARP) Syndrome (8993 hotspot/Test for mtATP6 gene sequence) *LTAT: 14 working days POLG-Related Disorders (POLG gene sequence) Mitochondrial DNA Depletion Syndromes (ANT1, DGUOK, MPV17, POLG, RRM2B ,SUCLA2, SUCLG1, TWINKLE, TYMP gene sequence) Multiple Respiratory Chain Deficiencies (Mitochondrial Translation Defect) (GFM1 gene sequence) Multiple Respiratory Chain Deficiencies (Mitochondrial Translation Defect) (GFM1 gene sequence) Whole mitochondrial DNA (hotspot and Full panel: mtDNA genes sequence & deletion/ duplication) Note: Request only by Clinical Geneticist/Neurologist.
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01
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18
GENETIC SYNDROMES
50. 51. 52.
Fragile X Syndrome (FRAXA: Expansion of CGG repeats of FMR1 gene & FRAXE Expansion of GCC repeats of FMR2 gene) Prader-Willi Syndrome (Test for Absence of non-methylated paternal copy of SNRPN exon 1/ promoter regions) Angelman Syndrome (Test for Absence of non-methylated maternal copy of SNRPN exon 1/ promoter regions and UBE3A gene sequence & deletion/duplication analysis)
53. 54. 55.
Alagilles syndrome (JAG1 gene sequence and deletion&duplication analysis and NOTCH2 gene sequence) Noonan Syndrome (PTPN11 gene sequence) Schinzel Giedion Syndrome (SETBP1 gene sequence)
NEUROGENETIC DISORDERS
56. 57.
Spinal Muscular Atrophy (SMN gene deletion, copy number & sequence) Primary Dystonia (THAP1 gene sequence)
58. 59.
MCT8-Specific Thyroid Hormone Cell Transporter Deficiency (SLC16A2 gene sequence) Lissencephaly (LIS1 gene sequence)
OTHER GENETIC DISORDERS
60. 61. 62. 63. 64. 65. 66. 67. 68.
Pseudorheumatoid Dysplasia (WISP3 gene sequence) Berardinelli Congenital Lipodystrophy (AGPAT2, BSCL2 gene sequence) Retinoblastoma (RB1 gene sequence & deletion/duplication) X-Chromosome Inactivation (Androgen receptor Exon 1 fragment analysis) Acute intermittent porpohyria (HMBS gene sequence & deletion/duplication) Alexander disease (GFAP gene sequence) Biotinidase Deficiency (BTD gene sequence) CADASIL (NOTCH3 Hotspot and Full gene Sequencing*) LTAT for full gene sequencing: 45 days Congenital Disorder of Glycosylation (CDG) Genotyping/ Phosphomannomutase 2 Deficiency (PMM2 gene sequence)
69. 70. 71. 72. 73. 74. 75. 76. 77. 78.
Early-onset Primary Dystonia (TOR1A gene sequence) Hypophosphatasia (ALPL gene sequence) Leopard Syndrome (PTPN11 gene sequence) Maroteaux-Lamy Syndrome, MPS VI (ARSB gene sequence) Medium Chain Acyl-CoA Dehydrogenase (ACADM gene sequence) Very Long Chain Acyl-CoA Dehydrogenase (ACADVL gene sequence) Metachromatic Leukodystrophy (MLD)/ Arylsulphatase A (ARSA gene sequence) PTEN-associated Diseases (PTEN gene sequence & deletion / duplication) Severe Congenital Neutropenia (ELANE gene sequence) DNA Extraction and Storage LTAT: 2 working days
19
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SENARAI UJIAN RESEARCH BASED DI UNIT BIOKIMIA, IMR. (TEMUJANJI DIPERLUKAN)
NO TESTS TYPE OF SPECIMEN
SPECIMEN CONTAINER VOLUME OF SPESIMEN REFERRAL LAB TURN AROUND TIME
1. Alpha-aminoadipic semialdehyde Urine Urine container 2 mls 15 working days
2. Alpha-fucosidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
3. Alpha-galactosidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
4. Alpha-hexosaminidase (Alpha-N-acetyl glucosaminidase)
Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
5. Alpha-iduronidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
6. Alpha-mannosidase, leucocytes Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
7. Alpha-mannosidase, plasma Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
8. Aryl sulphatase A Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
9. Arylsulphatase B (N-acetylgalactosamine 4-sulfatase)
Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
10. Aspartyl-glucosaminidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
11. Beta Hexosaminidase A Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
12. Beta Hexosaminidase A + B Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
13. Beta-galactosidase Whole blood 2 x 2.5 mls blood in EDTA tubes 15 working days
14. Beta-glucosidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
15. Beta-glucuronidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
16. Beta-mannosidase A+B (TOTAL) Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
17. Biogenic amines (5-HIAA, HVA, VMA) Urine Urine container 2mls 15 working days
18. Biogenic amines (5-HIAA, HVA, VMA, 3 MD) CSF Special container from IMR 2mls 15 working days
19. Carnitine, urine 24 hour urine 24 hour urine container 24 hours urine collection 10 working days
20. Creatine Urine, random Urine container 2 mls 15 working days
21. Galactocerebrosidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
22. Galactose-1-phosphate Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 10 working days
23. Galactose-6-sulphatase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
24. Glycogen branching enzyme Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
25. Iduronate-2-sulphatase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
26. N-acetylglucosamine-6-sulphatase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
27. Palmitoyl-protein thioesterase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
28. Pterins, urine Urine, random Urine container 5 mls 15 working days
29. Sugar & Polyols, urine Urine, random Urine container 5 mls 16 working days
30. Sulphamidase Whole blood EDTA 2 x 2.5 mls blood in EDTA tubes 15 working days
20
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SENARAI UJIAN DI JABATAN KIMIA
NO TESTS* (includes but not limited to) REFERRAL LAB TURN
AROUND TIME TYPES OF SPECIMEN/CONTAINER/SPECIMEN VOLUME
REMARK
1. Alcohol (ethanol) 1 – 2 Months Blood, urine, gastric content, gastric lavage content, vomitus, others.
Please consult officer in charge (toxicology unit), before sending samples. Jabatan Kimia Malaysia, Cawangan Johor: 07-222 6366/07-222 6569
2. Insecticide (organophosphate)
3. Drugs (Barbiturates, cocaine)
4. Corrosive agents
5. Gas
6. Heavy metals (Aluminium, arsenic, bromide, cadmium, chromium, lead, nikel, mercury, selenium, zinc)
7. Solvents
8. Others
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN-LAMPIRAN
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 1: SENARAI UJIAN STAT
SENARAI UJIAN STAT DI MAKMAL PATOLOGI KIMIA, HSA
BIL UJIAN LTAT
1 Arterial Blood Gas 30 minit
2 Ammonia 1 jam
3 Amylase 1 jam
4 Bilirubin 1 jam
5 Calcium 1 jam
6 Chloride 1 jam
7 Creatinine 1 jam
8 CSF chemistry 1 jam
9 Glucose 1 jam
10 Lactate 1 jam
11 Magnesium 1 jam
12 Paraquat (urine) 1 jam
13 Phosphate 1 jam
14 Potassium 1 jam
15 Renal Function test 1 jam
16 Sodium 1 jam
17 Troponin T 1 jam
18 Urea 1 jam
19 LFT* (untuk kes-kes acute liver failure, pre-eclampsia dan severe dengue sahaja)
1 jam
NOTA:
1. Ujian-ujian lain yang diperlukan STAT hendaklah dipohon melalui pegawai perubatan/pakar
patologi Kimia yang bertugas.
2. Sekiranya ujian STAT dipohon bersama dengan ujian-ujian lain (yang tiada dalam senarai STAT)
dalam satu spesimen atau ujian STAT dihantar secara batch, ujian tersebut akan dijalankan
secepat mungkin tetapi LTAT STAT mungkin tidak terjamin.
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 2: JADUAL PENGHANTARAN SPESIMEN KE MAKMAL RUJUKAN
1) Jadual penghantaran spesimen ke makmal rujukan.
MAKMAL RUJUKAN HARI PENGHANTARAN
Hospital Kuala Lumpur Isnin & Rabu
Institute of Medical Research Isnin & Rabu
Hospital Ampang Isnin & Rabu
Hospital Putrajaya Rabu
Jabatan Kimia Malaysia,
cawangan Johor
Khamis
Permai Mengikut permintaan
*Spesimen yang diterima selepas pukul 10 pagi pada hari penghantaran akan dihantar pada hari
penghantaran yang berikutnya
2) Semua permohonan hendaklah disertakan dengan borang PER.PAT 301, kecuali dinyatakan
sebaliknya.
3) Semua ujian yang di rujuk ke makmal di luar HSA dianggap ujian khas dan perlu tandatangan
pakar yang memohon dan sejarah pesakit yang lengkap. Borang yang tidak lengkap akan ditolak.
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 3: PANDUAN BEKAS SPESIMEN
TUBES COLOUR TUBES COLOUR
Lithium heparin Green
Urine container
Plain tube Red
24 hour urine container
Gold
K2-EDTA
Lavender
Bijou bottle
Fluoride Oxalate
Grey
Heparinized
syringe
Syringe must be
labeled.
Unlabeled syringe will
be rejected.
Whatman 903 filter
paper
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 4: RECOMMENDED ORDER OF DRAW FOR MULTIPLE TUBE COLLECTIONS
ORDER OF DRAW CLOSURE COLOR COLLECTION TUBE MIXED BY INVERTING
FIRST
LAST
Blood culture 8 to 10 times
Citrate tube 3 to 4 times
Or
Serum tube (with or without gel) 5 times
Heparin Tube 8 to 10 times
EDTA 8 to 10 times
Fluoride 8 to 10 times
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 5: PANDUAN UJIAN RENIN DAN ALDOSTERONE
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
Whatman 903 filter paper.
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
LAMPIRAN 6: BORANG-BORANG
BORANG PER. PAT 301
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
REQUEST FORM FOR MULTIPLE MYELOMA AND SPECIFIC PROTEIN (IMR/UMDP/01)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
REQUEST FORM FOR MOLECULAR DIAGNOSTIC SERVICES (IMR/UMDP/02) (PAGE 1 OF 3)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
REQUEST FORM FOR MOLECULAR DIAGNOSTIC SERVICES (PAGE 2 OF 3)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
REQUEST FORM FOR MOLECULAR DIAGNOSTIC SERVICES (PAGE 3 OF 3)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
IEM REQUEST FORM (PAGE 1 OF 2)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
IEM REQUEST FORM (PAGE 2 OF 2)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
BORANG UJIAN DADAH (UPD 1 PINDAAN)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
BORANG PERMOHONAN BAGI PEMERIKSAAN FORENSIK/TOKSIKOLOGI - JABATAN KIMIA (PAGE 1 OF 3)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
BORANG PERMOHONAN BAGI PEMERIKSAAN FORENSIK/TOKSIKOLOGI - JABATAN KIMIA (PAGE 2 OF 3)
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PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
BORANG PERMOHONAN BAGI PEMERIKSAAN FORENSIK/TOKSIKOLOGI - JABATAN KIMIA (PAGE 3 OF 3)
PANDUAN PERKHIDMATAN PATOLOGI KIMIA, HSA, VERSI 1, 2015
NOTA