Ihda Uswatun Shalihah Shohibuddin and Wan Wardatul Amani Wan Salim* Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Gombak 50728, Kuala Lumpur, Malaysia.

Email : uswah.solehah@gmail.com and asalim@iium.edu.my*

ABSTRACT

ACKNOWLEDGEMENT: The research work is funded by NanoSkunkworkX Sdn. Bhd through a Sponsored Research Grant Awarded to the Amani Research Group.

REFERENCES:

1. Soum et al., (2019). Programmable paper-based microfluidic devices for biomarker detection. Micromachines. 10 (8): 516. https://doi.org/10.3390/mi10080516

2. Park et al., (2017). An autonomous lab on a chip for space flight calibration of gravity-induced transcellular calcium polarization in single-cell fern spores.

Lab on a Chip. https://doi.org/10.1039/C6LC01370H

Microbotics CaLSens: NANOTECHNOLOGY-BASED CALCIUM SENSOR

FOR EARLY DETECTION OF PREGNANCY COMPLICATION

METHODOLOGY

Ca2+ level

1200 mg/day

13/2/2020

Patient A

Ca2+ level

600 mg/day

13/2/2020

Patient B

Microbotics CaLSens.

13/2/2020

Patient A

Patient C

Patient D

Patient B

1200

1500

1000

600

Hospital Information

System

Ca2+ ok

Go see

doctor

To develop a nanosensor dot

To characterize and evaluate the

developed sensor

OBJECTIVES

PROPOSED SOLUTION

PROBLEM STATEMENT

70 % 6 %

40 % Preterm delivery

Reduced bone calcium

content

Reduced fetal growth GLOBAL CALCIUM

DEFICIENCY

By developing a calcium sensor.

Microbotics CaLSens:

FINDINGS

COMMERCIALIZATION POTENTIAL

WHERE CAN WE GO NEXT?

The proposed nanotechnology-based calcium sensor provides an

initial insight for early detection of pregnancy complications by

constant monitoring of calcium ion in bodily fluids.

CONCLUSION

2018

USD 23.98 B

2026

USD 50.51 B

240 mV

scan starts

30 mV

scan starts

Δi = 0.965 mA

scan starts

1) SHIFT IN PEAK POTENTIAL, EP

2) PEAK

CURRENT,

IP

3) EP & IP

NOVELTY OF IDEA An nsD Technology

Commercialization potential

Point-of-care (POC)

Diagnostics Market Size CAGR

of 9.6 %

Future directions

with respect to

nsD Technology

A microfluidic

POC Device

-- negative control -- nsD 1 -- nsD 2 -- nsD (1 & 2)

nsD 1

nsD (1 & 2) nsD 2

0.030 0.030

0.240 0.180

0.281

0.076

1.041 0.998

0.000

0.200

0.400

0.600

0.800

1.000

1.200

SPCE rGO:PSS/SPCE PEDOT:PSS/SPCE rGO:PSS-PEDOT:PSS/SPCE

Shift in peak potential, Epa (V) Peak Current, Ip (mA)Ep

negative nsD 1 nsD 2 nsD (1& 2)

control

Hig

her

i p

Smaller shift in Ep

ip = 0.998 mA ip = 1.041 mA

Ep = 30 mV

Ep = 240 mV

ip = 0.076 mA

Ep = 180 mV

nsD 1

ARG Group

Member

Real-time

monitoring

Portable Calcium

sensing Miniaturization

nsD 2

Body fluid flow Sensor readout

We aim for a next-generation point-of-care (POC) device that is sensitive with high stimulus-responsive properties to monitor calcium levels during

pregnancy. The POC device consists of nanosensor dot (nSD) transduction technology that are 100 times more sensitive than a conventional

transducer used in a normal glucose meter. We have successfully characterized the nSD via electrochemical cyclic voltammetry (CV)

measurements where the CV graphs showed the nSD to have fast electron transfer kinetics at the electrode-electrolyte interface, illustrated by the

small shift in peak potential (Ep) and high peak current (Ip). Since the size of global blood testing market was estimated at USD 23.98 billion in 2018

and is expected to be doubled in 2026, the proposed technology has the potential to be commercialized. The nSD technology is funded by

NanoSkunkworkX Sdn Bhd through a Sponsored Research Grant awarded to the Amani Research Group, International Islamic University Malaysia.

Sample

inlet

Microfluidic

channel

Sample

outlet