LAPORAN PRAKTIKUM wahda

7/25/2019 LAPORAN PRAKTIKUM wahda

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LAPORAN PRAKTIKUM

BAKTERIOLOGI II

ISOLASI DAN IDENTIFIKASI BAKTERI STAPHYLOCOCCUS Sp PADA

SAMPEL MAKANAN DAN MINUMAN

Disusun ole!

N"#" ! $AHDA NURISMI

NIM ! %& '%&( &(' %')

Kelo#po* ! + ,LIMA-

PROGRAM STUDI DIII ANALIS KESEHATAN

STIKes MEGA RE.KY MAKASSAR

/0%(1/0%2


2/15

LEMBAR PENGESAHAN

3u4ul Pe56o7""n ! Isol"si 4"n i4en8i9i*"si B"*8e5i s8"p:lo6o66us Sp

p"4" s"#pel #"*"n"n 4"n #inu#"n

N"#" P5"*8i*"n ! $"4" Nu5is#i

NIM ! %& '%&( &(' %')

H"5i1T"n;;"l Pe56o7""n ! Sel"s" 2 o*8o7e5 /0%(

Kelo#po* ! + ,LIMA-

Re*"n Ke5


3/15

BAB I

PENDAHULUAN

A= LATAR BELAKANG

B"*8e5i "4"l" *elo#po* o5;"nis#e :"n; 8i4"* #e#ili*i #e#75"n in8i sel=

O5;"nis#e ini 8e5#"su* *e 4"l"# 4o#"in p5o*"5io8 4"n 7e5u*u5"n s"n;"8

*e6il ,#i*5os*opi*-? se58" #e#ili*i pe5"n 7es"5 4"l"# *ei4up"n 4i 7u#i=

B"*8e5i #e5up"*"n o5;"nis#e :"n; p"lin; 7"n:"*


4/15

pe#"n"s"n?8e5#"su* ;5"# posi8i9? #e#7en8u* spo5"? 7e5si9"8 "e5o7 s"#p"i

"n"e5o7 9"*ul8"8i9? 4"n 7"6illus *e7"n:"*"n "4"l" s"p5o9i8= M"sin;@#"sin;

7"6illus "n:" #en;"sil*"n s"8u spo5" :"n; 5esis8en 8e5"4"p pe5l">"n"n

suu? 5"4i"si?4esi*"si 4"n 4esin9e*8"n ,Mo"##"4?/00)-=

B= TU3UAN PERCOBAAN

%= un8u* #e#7u"8 #e4i" pe58u#7u"n p"4" 7"*8e5i

/= un8u* #en;i4en8i9i*"si #o59olo;i se58" si9"8 7"*8e5i Staphylococcus Sp

4en;"n


5/15

Du" #i*5oo5;"nis#e 8e54i5i 4"5i li#" *elo#po* o5;"nis#e

7"*8e5i? p5o8oo"? i5us? se5" "l;"e 4"n 6en4">"n #i*5os*opis= Ki8" #e#pel"


6/15

Pe>"5n""n G5"# "8"u #e8o4e G5"# "4"l" s"l" s"8u 8e*ni* pe>"5n""n

:"n; p"lin; pen8in; 4"n lu"s :"n; 4i;un"*"n un8u* #en;i4en8i9i*"si

*u#"n B"6illus sp= Pen;e6"8"n ;5"# 4i;un"*"n un8u* #en;i4en8i9i*"si

*u#"n B"6illus sp= B"*8e5i G5"# posi8i9 "*"n #e#pe58""n*"n "8 pe>"5n"

*5is8"l iole8 4"n *"5en"n:" "*"n 8"#p"* 7e5>"5n" un;u 8u" 4i 7">" #i*5os*op=

A4"pun 7"*8e5i ;5"# ne;"8i9 "*"n *eil"n;"n "8 pe>"5n" *5is8"l iole8 se8el"

4i6u6i 4en;"n "l*ool? 4"n se>"*8u 4i7e5i "8 pe>"5n" 8"n4in;"nn:" :"i8u 4en;"n

"8 pe>"5n" "i5 9u6sin "8"u s"95"nin "*"n 8"#p"* 7e5>"5n" #e5"= Pe57e4""n

>"5n" ini 4ise7"7*"n ole pe57e4""n 4"l"# s85u*8u5 *i#i">i 4in4in;

seln:"= B"*8e5i ;5"# posi8i9 4in4in; seln:" 8e5susun ole pep8i4o;li*"n 4"l"#


7/15

Me4i" ";"5 #e5up"*"n su7s85"8 :"n; s"n;"8 7"i* un8u* #e#is"*"n

6"#pu5"n #i*5oo5;"nis#e sein;;" #"sin;@#"sin; "5n" pu8i s"#p"i

*unin;? 8e8"pi "4" 7e7e5"p" spesies :"n; #e#7e5i*"n >"5n" 8e5sen4i5i=


8/15

BAB III

METODE KER3A

A= $AKTU DAN TEMPAT

P"4" P5"*8i*u# isol"si 4"n i4en8i9i*"si B"*8e5i s8"p:lo6o66us Sp p"4"

7""n #"*"n"n 4"n #inu#"n 4"5i ">"l p5"*8i*u# #ul"i 4"5i pe#7u"8"n

#e4iu#? isol"si? pen;e6"8"n G5"# 4"n U


9/15

= KIA

5= Me4i" ;ul"@;ul" ,l"*8os"?su*5os"?;lu*os"?4"n #"l8ose-

s= SIMC= PROSEDUR KER3A

%= Isol"si *oloni

"= Kul8u5is"si1pe#7i"*"n *oloni

Disi"p*"n Al"8 4"n 7""n :"n; "*"n 4i;un"*"n

Dien6e5*"n s"#pel #inu#"n ,"i5 ;"llon- in;;" pen;en6e5"n %0@'

Ke#u4i"n 4ipipe8 0?% #l s"#pel 8e5se7u8? l"lu 4i#"su**"n *e4"l"#

#e4i" BA ,Bloo4 A;"5- 4"n NA ,Nu85ien A;"5-=

Dise7"5 "8"u 4ii;@"; s"#pel p"4" #e4i" 4en;"n #en;;un"*"n

ose :"n; 8el" 4ipi"5n""n G5"# un8u* #eli"8 "5n""n G5"#

Di7u"8 p5ep"5"8 4en;"n #ensuspensi*"n *oloni 4en;"n "u"4es

s8e5il

Ke#u4i"n 4i9i*s"si 4i"8"s "pi Bunsen

Di8"#7"*"n %@/ 8e8es K5is8"l iole8? l"lu 4i"#*"n sel"#" ' #eni8

Se8el" *e5in;? *e#u4i"n 4i6u6i "i5 #en;"li5

Di8"#7"*"n %@/ 8e8es lu;ol? l"lu 4i4i"#*"n sel"#" / #eni8

Di6u6i "i5 #en;"li5

De*olo5is"si 4en;"n "l6ool 2 sel"#" / #eni8

Di6u6i "i5 #en;"li5

Di8"#7"*"n %@/ 8e8es "i5 9u6sin sel"#" % #eni8

Di6u6i "i5 #en;"li5

Di*e5in;*"n 4"n 4i"#"8i p"4" #i*5os*op 4en;"n pe57es"5"n

o7


10/15

7- Di8e8es*"n l"5u8"n H/O/"8"u Hi45o;en pe5o*si4"=

6- Di"#"8i "4" "8"u 8i4"*n:" ;ele#7un; p"4" *oloni=

/= U


11/15

BAB I+

HASIL DAN PEMBAHASAN

%= HASIL PENGAMATAN

/= PEMBAHASAN

P5insip isol"si 7"*8e5i "4"l" #e#is"*"n su"8u #i*5o7" 4"5i

#i*5o7" l"inn:" sein;;" 4ipe5ole *ul8u5 #u5ni=P5oses isol"si ini

#en"5n""n :"n; p"lin; pen8in; 4"n

lu"s :"n; 4i;un"*"n un8u* #en;i4en8i9i*"si *u#"n s8"p:lo6o66us sp=

Pe>"5n""n ;5"# 4il"*u*"n 4en;"n 6"5" #e#7u"8 p5ep"5"8 4en;"n

#ensuspensi*"n *oloni 4en;"n Au"4es s8e5il :"n; 4i9i*s"si 4i"8"s "pi

Bunsen se8el" i8u 4i*e5in;*"n= Se8el" *e5in;? 4i8"#7"*"n K5is8"l;en8i"n iole8 s"#p"i #enu8upi pe5#u*"""n sel"#" /@' #eni8 4"n 6u6i "i5

#en;"li5= Di8"#7"*"n lu;ol 4i"#*"n sel"#" / #eni8 6u6i l";i "i5

#en;"li5= Ke#u4i"n 4e*olo5is"si 4en;"n "l6ool 2 sel"#" / #eni8 4"n

6u6i "i5 #en;"li5= *e#u4i"n 4i8"#7"*"n l";i "i5 9u6sin sel"#" % #eni8

4"n 4i6u6i "i5 #en;"li5= *e5in;*"n 4"n *e#u4i"n 4i"#"8i 4i7">"

#i*5os*op 4en;"n pe57es"5"n &0 4"n %00 , 8"#7"*"n oil e#e5si-=

Se8el" 4i"#"8i 4i7">" #i*5os*op? 4i4"p"8*"n "sil :"n; ne;"8i9

:"i8u 7e57en8u* 7"sil 4"n 7e5>"5n" #e5"= Se8el" i8u 4il"n


12/15

u"5n" *unin; 8u" :"n; *e#u4i"n 4ipin4"*"n 4i*e58"s s"5in; 4"n 4i8e8esi

l"5u8"n o*si4"se se7"n:"* % 8e8es= Se8el" 4i8e8esi 4i4"p"8*"n "sil :"n; posi8i9

*"5en" #e#7e5i*"n "sil :"n; 7e5>"5n" i8"#=

Se8el" u"5n" *unin;= Ke#u4i"n 4il"*u*"n pe#is""n p"4" MR

4"n +P 4i#"n" MR 4i8"#7"*"n 4en;"n pe5e"*si #e8:l Re4 :"n; #e#7e5i*"n

"sil :"n; ne;"8i9 *"5en" 8i4"* 8e5"5n" *unin; se4"n;*"n p"4" #e4i" +P :"n; 4i8"#7"*"n pe5e"*si KOH

'0 4"n @ N"98ol "5n" #e5" #u4" un8u* #en4"p"8*"n "sil :"n; posi8i9=

Ke#u4i"n se8el" 4il"*u*"n u


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8e5se7u8 4i8"n4"i 4en;"n 8i4"* 8e5"5n" p"4" #e4i"

:"n; 8e8"p 7e5>"5n" #e5"=

Se8el" i8u 4il"n"5n" *unin; 8e8"p=

"4"n:" sul9u5 p"4" #e4i" 8i4"* 8e5


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DAFTAR PUSTAKA

88p!11nlo"4s1')0@)&/2@%@PB/0,%-=p49
http://jurnal-mikrobiologi.blogspot.com/2010/12/isolasi-dan-identifikasi-bakteri_20.htmlhttp://jurnal-mikrobiologi.blogspot.com/2010/12/isolasi-dan-identifikasi-bakteri_20.htmlhttp://c/Users/personal/Downloads/3780-7426-1-PB%20(1).pdfhttp://c/Users/personal/Downloads/3780-7426-1-PB%20(1).pdfhttp://jurnal-mikrobiologi.blogspot.com/2010/12/isolasi-dan-identifikasi-bakteri_20.htmlhttp://jurnal-mikrobiologi.blogspot.com/2010/12/isolasi-dan-identifikasi-bakteri_20.html


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I5i"n8o Koes? /0%&= B"*8e5iolo;i? Mi*olo;i? 4"n +i5olo;i= Pene57i8 ALFABETA !

B"n4un;=

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