P3 Johor 2010

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4551/3 Hak Cipta JPNJ 2010 [Lihat sebelah

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1 ml of saliva solution1 ml larutan air liur

Answerall questionsJawab semua soalan

1. A group of student carried out an experiment to study the effect of temperature onthe rate of amylase reaction on starch.Sekumpulan pelajar menjalankan eksperimen untuk mengkaji kesan suhu terhadap

kadar tindakbalas amilase terhadap kanji.

Diagram 1 shows the apparatus set-up of the experiment.Rajah 1 menunjukkan susunan radas untuk eksperimen tersebut.

Step 1 : 1ml of saliva solution and 5 ml of 1% starch suspension were placed intwo different test tube in a water bath at 5 C for 5 minute.

Langkah 1 : 1ml larutan air liur dan 5 ml 1% ampaian kanji diletakkan ke dalam duatabung uji yang berbeza di dalam kukus air 5 C selama 5 minit.

Step 2 : After 5 minute the saliva solution was added to the starch suspension.Langkah 2 : Selepas 5 minit larutan air liur itu dimasukkan ke dalam ampaian kanji

tersebut.

Step 3 : At one minute intervals two drop of the mixture was withdrawn witha dropper and placed on a drop of iodine solution on a white tile.

Langkah 3 : Dalam selang masa satu minit, dua titis campuran itu di ambilmenggunakan penitis dan dititiskan pada satu titik larutan iodin di atas

jubin putih.

Step 4 : Step 3 was repeated until the blue black solution turn into yellowish.The time taken is recorded.

Langkah 4 : Langkah 3 diulang sehingga warna larutan biru gelap bertukarkekuningan. Masa yang diambil dicatatkan.

Step 5 : Step 1 to 5 is repeated using water baths at 5 C, 28 C,37 C , 45 Cand 55 C.

Langkah 5 : Langkah 1 hingga langkah 4 di ulang dengan menggunakan suhukukus 5 C, 28 C,37 C , 45 C dan 55 C.

Diagram 1Rajah 1

iceais

5 ml of 1% starch suspension5 ml 1% ampaian kanji

waterair

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(a) Record the time taken for the iodine solution remains yellowish in the boxesprovided in Table 1.Rekodkan masa yang di ambil untuk warna iodine kekal kekuningan di dalamkotak yang disediakan dalam Jadual 1. [ 3 marks]

[3 markah]

TemperatureSuhu ( C)

Time taken for iodine solution remains yellowish / (minute)Masa yang diambil untuk warna iodine kekal kekuningan/ (minit)

5

28

37

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Table 1Jadual 1

45

55

3

1 a

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(b) (i) State two different observations made from Table 1.Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 1.

Observation 1 /Pemerhatian 1 :

__________________________________________________________

__________________________________________________________

__________________________________________________________

Observation 2 /Pemerhatian 2:

__________________________________________________________

__________________________________________________________

__________________________________________________________[ 3 marks][3 markah]

(ii) State the inference which corresponds to the observations in 1 (b) (i).Nyatakan inferens yang sepadan dengan pemerhatian di 1 (b) (i).

Inference from observation 1 /Inferens daripada pemerhatian 1 :

__________________________________________________________

__________________________________________________________

__________________________________________________________

Inference from observation 2 /Inferens daripada pemerhatian 2 :

__________________________________________________________

__________________________________________________________

__________________________________________________________[ 3 marks][3 markah]

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1(b)(

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(c) Complete Table 2 based on this experimentLengkapkan Jadual 2 berdasarkan eksprimen ini

VariablePembolehubah

Method to handle the variableCara mengendali pembolehubah

Manipulated variable

Pembolehubah dimanipulasi

________________________

________________________

________________________

________________________

________________________

________________________

Responding variablePembolehubah bergerakbalas

________________________

________________________

________________________

________________________

________________________

________________________

Constant variablePembolehubah dimalarkan

________________________

________________________

________________________

________________________

________________________

________________________

Table 2Jadual 2

[ 3 marks][3 markah]

(d) State the hypothesis for this experiment.Nyatakan hipotesis bagi eksperimen ini.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________


3

1(c)

3

1 d

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(e) (i) Construct a table and record all the data collected in this experiment.Bina satu jadual dan rekodkan semua data yang dikumpul dalam eksperimenini.

Your table should have the following title.

Jadual anda hendaklah mengandungi tajuk-tajuk berikut.`

TemperatureSuhu

Time taken for iodine solution remains yellowishMasa yang di ambil untuk warna iodin kekal kekuningan

Rate of enzyme reactionKadar tindakbalas enzim


(ii) Use the graph paper provided on page 8 to answer this part ofquestion. Using the data in 1(e)(i), draw the graph of the rate ofenzyme reaction against the temperature.Gunakan kertas graf yang disediakan dalam di mukasurat 8 untukmenjawab soalan bahagian ini. Menggunakan data 1(e)(i), lukiskangraf kadar tindakbalas enzim melawan suhu.


3

1(e

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(f) Based on the graph in 1 (e) (ii) , explain the relationship between rate of enzymereaction and temperature .Berdasarkan kepada graf di 1 (e) (ii), terangkan hubungan antara kadartindakbalas enzim dengan suhu.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________


(g) This experiment is repeated by increasing the concentration of the salivasolution. Predict the time taken for iodine solution remains yellowish.

Explain your prediction.Eksperimen ini diulang dengan menambah kepekatan larutan air liur. Ramalkanmasa yang di ambil untuk warna iodine kekal kekuningan.Terangkan ramalan anda.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________

[ 3 marks]

[3 markah]

(h) Based on the result from the experiment what can be deduced about enzyme.Berdasarkan keputusan daripada eksperimen ini, apakah yang dapat dirumuskantentang enzim.

_______________________________________________________________

_______________________________________________________________

_______________________________________________________________


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1(f)

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(i) The following is a list of enzyme and medium for reaction.Berikut ialah senarai enzim dan medium untuk bertindakbalas.

Classify the enzyme with the suitable medium for reaction in Table 3.Klasifikasikan enzim tersebut dengan medium tindakbalas yang sesuai didalam Jadual 3.

Table 3Jadual 3


EnzymeEnzim

Medium for reactionMedium untuk bertindakbalas

Alkaline Trypsin AmylaseBeralkali Tripsin Amilase

Pepsin Neutral AcidicPepsin Neutral Berasid

3

1(i)

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2. A group of students carry out an experiment to study the effect of environmentalfactor on the rate of transpiration of a balsam plant.Sekumpulan pelajar menjalankan eksperimen untuk mengkaji kesan faktor

persekitaran terhadap kadar transpirasi pokok keembong.

Based on the above information, plan a laboratory experiment to determine the

effect of the temperature on the rate of transpirationBerdasarkan maklumat di atas , rancang experiment dalam makmal untukmengkaji kesan suhu terhadap kadar transpirasi

The planning of your experiment must include the following aspects:Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:

. The planning of your experiment must include the following aspects:Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:

y Problem statementPenyataan masalah

y Objective of investigationObjektif kajian

y VariablesPembolehubah-pembolehubah

y HypothesisHipotesis

y List of materials and apparatusSenarai bahan dan radas digunakan

y Technique usedTeknik yang digunakan

y Experimental procedure or methodKaedah atau prosedur eksperimen

y Presentation of dataCara data dipersembahkan

y ConclusionKesimpulan

END OF QUESTION PAPER

KERTAS SOALAN TAMAT

[17 marks][17 markah]

P3 Johor 2010

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